Pieter C. Dorrestein, Ph.D.
Skaggs School of Pharmacy and Pharmaceutical Sciences
Departments of Pharmacology, Chemistry and Biochemistry
Member of the Moores Cancer Center and Center for Marine Biotechnology and Biomedicine
The Dorrestein laboratory aims to develop new mass spectrometry approaches to detect and characterize therapeutic leads as well as their biosynthesis. The research in my lab falls into 4 areas: (1) The functional characterization of novel post-translational modifications involved in the biosynthesis of therapeutics or therapeutic targets. (2) Develop new methods to characterize metabolic exchange factors from microbial systems involved in cell-to-cell communication. All cells communicate with other cells. Currently, there are no tools to systematically study the molecular output of a small population of cells. My lab is developing mass spectrometry based approaches to study the universal phenomenon of cell-to-cell communication to discover new biological modulators. (3) Therapeutic target identification, including off-targets. Target identification is very important to the therapeutic discovery pipeline. This is done in collaboration with other scientists at UCSD, UCSC, SALK, SIO and elsewhere. (4) Monitoring the global response of therapeutics by monitoring the signaling proteome. While a therapeutic may have one or a few targets, the entire proteome of a cell or tissue will be affected. The phosphoproteome provides insight into the effect therapeutics have on cells or tissues and is monitored upon therapeutic stimulation providing insights into key regulatory pathways.
Education: B.A. in Chemistry (1999) Northern Arizona University; Ph.D. in Chemical Biology (2004) Cornell University; NRSA fellowship in Bioanalytical Chemistry (2006) University of Illinois.
Awards and Honors: ACACC award by Lilly in Analytical Chemistry (2008); V-foundation Scholar (2008); Featured in the journal Scientist as a “Scientist to Watch” (2008); Beckman Young Investigator (2008); Pharmaceutical Research and Manufacturers of America Research Award (2008).
Leadership Experience: Established the “Mass Spectrometry in Medicine and Biology group” at UCSD.
Lee et al. (2008). Discovery of a widely distributed toxin biosynthetic gene cluster. Proc. Natl. Acad. Sci. 105:5879-84.
Zhang et al. (2008). A phosphopantetheinylating polyketide synthase producing a linear polyene to initiate enediyne antitumor antibiotic biosynthesis. Proc. Natl. Acad. Sci. 5:1460-1465.
Yang et al. (2009). Translating metabolic exchange with imaging mass spectrometry. Nature Chemical Biology. 5:885-887.
Hughes et al. (2009). Marinopyrrole A Target Elucidation by Acyl Dye Transfer. Journal of the American Chemical Society. 131:12094-12096.
Ng et al. (2009). Dereplication and de novo sequencing of nonribosomal peptides. Nature Methods. 6:596-599.