University of California, San Diego | Skaggs School of Pharmacy and Pharmaceutical Sciences

RESOURCES

Protease Pathways

IPS - International Proteolysis Society scientists working on all aspects of proteolytic enzymes, their substrates, and inhibitors
MEROPS database peptidase and inhibitor database
UCSD Neuroscience Microscopy Facility at UCSD Fluorescence Cell Imaging

Enzymes: Biochemical Properties

Brenda database

Mass Spectrometry

Principals of Mass Spec Quantitation Principals of Mass Spectrometry quantitation
Scripps Center for MS providing analyses on a broad range of biological species from small molecules to large biological complexes

Bioinformatics

NCBI - National Center for Biotechnology Information National Center for Biotechnology Information
Agilent - Spectrum Mill Protein and peptide identification software
Thermo Finnigan - SEQUEST Protein and peptide identification software
Matrix Sciences - MASCOT Protein and peptide identification software

Organizations and Education

School of Medicine, UCSD School of Medicine
School of Pharmacy, UCSD School of Pharmacy
Neurosciences, UCSD Neurosciences Department
SFN - Society for Neurosciences Society for Neurosciences
HUPO - Human Proteome Organization Human Proteome Organization
NIH - National Institutes of Health National Institutes of Health

Recent Publications:

Hook, V., Yoon, M., Mosier, C., Ito, G., Podvin, S., Head, B.P., Rissman, R., O'Donoghue, A.J., Hook, G. (2020) Cathepsin B in neurodegeneration of Alzheimer's disease, traumatic brain injury, and related brain disorders. Biochim Biophys Acta Proteins Proteom. 1868, 140428.

Boutté, A.M., Hook, V., Thangavelu, B., Sarkis, G.A., Abbatiello, B.N., Hook, G., Jacobsen, J.S., Robertson, C.S., Gilsdorf, J., Yang, Z., Wang, K.K.W., Shear, D.A. (2020) Penetrating Traumatic Brain Injury Triggers Dysregulation of Cathepsin B Protein Levels Independent of Cysteine Protease Activity in Brain and Cerebral Spinal Fluid. J Neurotrauma 37(13),1574-1586.

Podvin, S., Jones, A., Liu, Q., Aulston, B., Ransom, L., Ames, J., Shen, G., Lietz, C.B., Jiang, Z., O'Donoghue, A.J., Winston, C., Ikezu, T., Rissman, R.A., Yuan, S., Hook, V. (2020) Dysregulation of Exosome Cargo by Mutant Tau Expressed in Human-induced Pluripotent Stem Cell (iPSC) Neurons Revealed by Proteomics Analyses. Mol Cell Proteomics 9, 1017-1034.

Yoon, M.C., Solania, A., Jiang, Z., Christy, M.P., Podvin, S., Mosier, C., Lietz, C.B., Ito, G., Gerwick, W.H., Wolan, D.W., Hook, G., O'Donoghue, A.J., Hook, V. (2021) Selective Neutral pH Inhibitor of Cathepsin B Designed Based on Cleavage Preferences at Cytosolic and Lysosomal pH Conditions. ACS Chem Biol. 16, 1628-1643.

Jiang, Z., Lietz, C.B., Podvin, S., Yoon, M.C., Toneff, T., Hook, V., O'Donoghue, A.J. (2021) Differential Neuropeptidomes of Dense Core Secretory Vesicles (DCSV) Produced at Intravesicular and Extracellular pH Conditions by Proteolytic Processing. ACS Chem Neurosci. 12, 2385-2398.

Campeau, A., Mills, R.H., Stevens, T., Rossitto, L.A., Meehan, M., Dorrestein, P., Daly, R., Nguyen, T.T., Gonzalez, D.J., Jeste, D.V., Hook V. (2021) Multi-omics of Human Plasma Reveals Molecular Features of Dysregulated Inflammation and Accelerated Aging in Schizophrenia. Molecular Psychiatry Nature 2021 Nov 5.

Podvin, S., Jones, A., Liu, Q., Aulston, B., Mosier, C., Ames, J., Winston, C., Lietz, C.B., Jiang, Z., O'Donoghue, A.J., Ikezu, T., Rissman, R.A., Yuan, S.H., Hook, V. (2021) Mutant Presenilin 1 Dysregulates Exosomal Proteome Cargo Produced by Human-Induced Pluripotent Stem Cell Neurons. ACS Omega 6(20), 13033-13056.

Boyarko, B., Hook, V. (2021) Human tau isoforms and proteolysis for production of toxic tau fragments in neurodegeneration. Frontiers in Neuroscience 15, 1-19.

Ashhurst, A.S., Tang, A.H., Fajtová, P., Yoon, M.C., Aggarwal, A., Bedding, M.J., Stoye, A., Beretta, L., Pwee, D., Drelich, A., Skinner, D., Li, L., Meek, T.D., McKerrow, J.H., Hook, V., Tseng, C.T., Larance, M., Turville, S., Gerwick, W.H., O'Donoghue, A.J., Payne, R.J. (2021) Potent Anti-SARS-CoV-2 Activity by the Natural Product Gallinamide A and Analogues via Inhibition of Cathepsin L. J Med Chem. 2021 Nov 3